Antioxidant and immunomodulatory activities of Rehmannia glutinosa extracts obtained with complex enzymes and ultrasonic wave

作者：Zichao Wang, Zhihan Shu, Yuxuan Zhang, Changxing Liu, Tao Liu, Hongtao Zhang, Yingjie Feng, Xueyi Qiao, Danye Zhu, Dandan Liu, Qi Wang, Qin Chen, Lemei An · 发表于：Preparative Biochemistry & Biotechnology · 年份：2025 · DOI：10.1080/10826068.2025.2562317 · 被引用次数：4 · 研究领域：Seaweed-derived Bioactive Compounds、Microbial Metabolites in Food Biotechnology、Polysaccharides and Plant Cell Walls

The comprehensive exploration and utilization of plants with both medicinal and culinary applications are crucial for human disease prevention and health maintenance. In this study, extracts of Rehmannia glutinosa were prepared using a complex enzyme- and ultrasound-assisted method. Bioactivity assays revealed that the R. glutinosa extracts exhibited potent radical-scavenging capabilities against DPPH, ABTS, hydroxyl, and superoxide radicals, with scavenging rates of 88.46% ± 1.35%, 98.90% ± 1.68%, 64.79% ± 1.12%, and 72.96% ± 1.46%, respectively. Furthermore, the extracts significantly protected RAW 264.7 cells from H2O2-induced oxidative damage, mitigating its effects and repairing cellular integrity. This was achieved by enhancing the activities of endogenous antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), while concurrently reducing malondialdehyde (MDA) levels. Additionally, the R. glutinosa extracts stimulated the secretion of immune factors (TNF-α, IL-1β, IL-6, and NO) in RAW 264.7 cells in a dose-dependent manner. Although the specific bioactive compounds within the extracts were not isolated or identified in this work, these findings collectively demonstrate the potential of R. glutinosa extracts as a valuable source of natural antioxidant and immunomodulatory ingredients.