Clinical relevance of circulating tumor DNA in HER2-positive advanced gastric cancer: a collaborative study of a phase Ib trial of dual HER2 and PD-1 targeted therapy (Ni-High)

作者：Hiroki Osumi, Takeru Wakatsuki, Akira Ooki, Keisho Chin, Hirokazu Shoji, Mariko Ogura, Izuma Nakayama, Noriko Yamamoto, Hidekazu Hirano, Hiroki Hara, Keiko Minashi, Eiji Shinozaki, Ken Kato, Naoki Ishizuka, Shigehisa Kitano, Kengo Takeuchi, Narikazu Boku, Kensei Yamaguchi, Daisuke Takahari · 发表于：Therapeutic Advances in Medical Oncology · 年份：2025 · DOI：10.1177/17588359251367344 · 被引用次数：7 · 研究领域：Cancer Genomics and Diagnostics、Cancer Cells and Metastasis、HER2/EGFR in Cancer Research

Background: The combination of anti–programmed cell death-1 antibody with human epidermal growth factor receptor 2 (HER2)–targeted therapy and chemotherapy is widely used in the United States and Europe for HER2-positive advanced gastric cancer (AGC). Molecular profiles that predict the efficacy of this dual-target therapy are unclear. Objectives: To explore the clinical utility of circulating tumor DNA (ctDNA) as a predictive marker of the efficacy of standard chemotherapy plus HER2 and programmed death-ligand 1 dual-targeted therapy in patients with HER2-positive AGC. Design: Collaborative study of the Ni-High phase Ib clinical trial. Methods: A total of 21 patients with tissue-confirmed HER2-positive AGC who received chemotherapy with dual-targeted therapy (capecitabine/S-1, oxaliplatin, trastuzumab, and nivolumab) in a phase Ib clinical trial (UMIN000034222) were enrolled. The association of genomic profiles in plasma ctDNA with tissue HER2 amplification status and their correlation with clinical outcomes was investigated. Results: Among the 21 patients studied, 20 (95.2%) showed somatic alterations in ctDNA. ERBB2 amplifications and single-nucleotide variants (SNVs)/indels were found in 12 (57.1%) and 3 (14.3%) patients, respectively. Significant associations between maximum mutant allele frequency (mMAF) and tumor size and between ctDNA and tissue ERBB2 copy numbers were found. Patients without ERBB2 SNV/indels showed longer median progression-free survival (PFS) and ov...