Novel antitubercular agents based on 2,4-disubstituted 5-(aryl-2-ylmethyl)-5H-pyrrolo[3,2-d]pyrimidines

作者：Vladimír Finger, Martin Vrbický, Ľubica Múčková, Lukáš Prchal, Martin Novák, Jan Marek, Ondřej Soukup, Michaela Hympánová, Ales Sorf, Markéta Benková, Jan Bartáček, Pavel Drabina, Martin Kufa, Ondrej Kovar, Lenka Fikejzlová, Martin Hrubý, Oksana Ozhelevska, Johannes Jagob, Jana Žďárová Karasová, Jitka Odvarkova, Iveta Kaderavková, Tomáš Rozsypal, Katarzyna Ewa Greber, Krzesimir Ciura, Jiří Janoušek, Martin Krátký, Ivone C. Igreja, Pavel Boštík, Radek Sleha, Jaroslav Roh, Jan Korábečný · 发表于：Biomedicine & Pharmacotherapy · 年份：2025 · DOI：10.1016/j.biopha.2025.118537 · 被引用次数：2 · 研究领域：Synthesis and Reactivity of Heterocycles、Synthesis and Reactions of Organic Compounds、Synthesis and biological activity

Tuberculosis (TB), caused by Mycobacterium tuberculosis ( Mtb ), remains a global health challenge, especially with the rise of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains. Current treatment regimens are prolonged and associated with significant toxicity, underscoring the need for novel therapeutic agents. This study investigates a new series of 2,4-disubstituted 5-(aryl-2-ylmethyl)-5 H -pyrrolo[3,2- d ]pyrimidine derivatives as potential antitubercular agents. The most promising compound, 74 , exhibited potent anti-TB activity, including against MDR strains, with a MIC 99 of 2 µM. Structure-activity relationship studies identified critical substitutions at positions 2- and 4- of the core scaffold that enhanced antimycobacterial potency, while bulkier aromatic moieties at position 5- were preferred. Despite its high efficacy, 74 demonstrated significant cytotoxicity, inhibition of cytochrome P450 enzymes and cardiotoxicity through hERG channel inhibition, highlighting challenges in further development. Pharmacokinetic studies of 74 revealed favorable systemic exposure with a prolonged half-life, suggesting its potential for less frequent dosing. Nonetheless, in vitro assays demonstrated rapid metabolic turnover, likely due to high intrinsic clearance, and the compound's elevated logD values further indicate the need for structural modifications to improve both solubility and metabolic stability. Efforts to introduce more polar substituents at the 4-...