Salmonella produces sulfide to compete with Escherichia coli in the gut lumen

作者：Anaïs Larabi, Connor R. Tiffany, Hugo L. P. Masson, Henry Nguyen, Eli J. Bejarano, Megan J. Liou, Lauren C. Radlinski, Aurore Demars, Renée M. Tsolis, Andreas J. Bäumler · 发表于：Proceedings of the National Academy of Sciences · 年份：2025 · DOI：10.1073/pnas.2504095122 · 被引用次数：6 · 研究领域：Gut microbiota and health、Salmonella and Campylobacter epidemiology、Sulfur Compounds in Biology

Hydrogen sulfide production is a characteristic that distinguishes Salmonella serovars from closely related species, such as Escherichia coli , but its biological significance remains obscure. Here, we show that PhsABC and AsrABC-mediated hydrogen sulfide production by Salmonella enterica serovar ( S. ) Typhimurium was linked to an increased abundance of Deltaproteobacteria and inhibition of cytochrome bd oxidase-mediated aerobic respiration of Escherichia coli in the murine large intestine. Functional phsABC and asrABC operons provided a growth benefit to S. Typhimurium in the cecum of Enterobacterales- free conventional mice only upon inoculation with commensal E. coli. In gnotobiotic mice engrafted with a defined community of 17 human Clostridia isolates, S. Typhimurium infection inhibited cytochrome bd oxidase-mediated aerobic respiration in E. coli only in the presence of Desulfovibrio piger , a sulfide-producing representative of the Deltaproteobacteria. A S. Typhimurium strain deficient for hydrogen sulfide production ( phsA asrA mutant) did not inhibit cytochrome bd oxidase-mediated aerobic respiration in E. coli , even when D. piger was present. Collectively, these data suggest that the phsABC and asrABC operons of Salmonella serovars provide a benefit during competition with closely related bacteria, such as E. coli , by inhibiting cytochrome bd oxidase-mediated aerobic respiration of the commensal.