Mechanistic Characterization of a Terpene Synthase for a Diterpene Diol from Chitinophaga jiangningensis

作者：Georges Bellier Tabekoueng, Bernd Goldfuß, Jeroen S. Dickschat · 发表于：Organic Letters · 年份：2025 · DOI：10.1021/acs.orglett.5c03451 · 被引用次数：5 · 研究领域：Plant biochemistry and biosynthesis、Natural product bioactivities and synthesis、Microbial Natural Products and Biosynthesis

is described that produces the diterpene diol jiangning-3,15-diol. The cyclization mechanism toward its tricyclic skeleton bearing one additional hydroxyl group as referred to usual type I diterpene synthase products was deeply investigated by isotopic labeling experiments and DFT calculations. The absolute configuration was established by chemical correlation using stereoselectively deuterated substrates.