Efficacy of Intense Pulsed Light AOPT ‐ LTL Technique in the Treatment of Melasma: An In Vivo and Clinical Study

作者：Jing Wang, Xinlan Wang, Li Gu, Zhinan Shi, Zhiyi Xu, Siqi Shen, Li‐Qun Gu, Lin Chen, Linling Ju, Chenglong Jin, Bingrong Zhou, Hui Hua · 发表于：Journal of Cosmetic Dermatology · 年份：2025 · DOI：10.1111/jocd.70443 · 被引用次数：4 · 研究领域：Dermatologic Treatments and Research、Laser Applications in Dentistry and Medicine、melanin and skin pigmentation

PURPOSE: To evaluate the efficacy and underlying mechanism of advanced optimal pulse technology intense pulsed light (AOPT) in low-energy triple-pulse long-width mode (AOPT-LTL) for melasma treatment. METHODS: An in vivo guinea pig model of melasma was established through progesterone injection and ultraviolet B radiation. Three sessions of AOPT-LTL treatment were performed weekly. The mRNA levels of key melanogenic enzymes, inflammatory factors, pro-angiogenic cytokines, and collagenolytic proteases were detected by qRT-PCR. Protein levels of stem cell factor (SCF) and mast cell growth factor receptor (c-KIT) were detected by immunofluorescence staining and Western blotting. Twenty melasma patients were treated with three sessions of AOPT-LTL treatment monthly, evaluated by the Modified Melasma Area and Severity Index (mMASI) and Erythema Index (EI). RESULTS: In guinea pigs with melasma, AOPT-LTL treatment effectively mitigated pigmentation and suppressed the expression of key genes involved in melanin production. There was a reduction in mast cell infiltration, decreased expression of inflammatory factors and pro-angiogenic factors, inhibition of angiogenesis, and alleviation of skin photoaging. Furthermore, AOPT-LTL also diminished the expression level of the SCF/c-KIT ligand/receptor pathway, which is closely associated with mast cell proliferation and activation. Consistently, three sessions of AOPT-LTL treatment effectively reduced skin melanin content, erythema severit...