Microbial community mediated by microbial agents improves the quality of Epimedium pubescens Maxim.

作者：Kunyang Lai, Xiufu Wan, Jiancai Xiao, Hongyang Wang, Shangxuan Shi, Binbin Yan, Chao-Geng Lyu, Chengcai Zhang, Yufei Zhang, Feng Yuan, Zhe Zhao, Shou-Dong Zhu, Chuanzhi Kang, Yan Zhang · 发表于：Science of Traditional Chinese Medicine · 年份：2025 · DOI：10.1097/st9.0000000000000078 · 被引用次数：5 · 研究领域：Medicinal Plant Pharmacodynamics Research、Fungal Biology and Applications、Ginseng Biological Effects and Applications

Background: Optimizing cultivation techniques for traditional Chinese medicine has become a crucial means to improve the quality of medicinal materials. Microbial agents, as environmentally friendly and efficient plant growth promoters and soil conditioners, have increasingly attracted attention in eco-agriculture research. Objective: Our understanding remains limited regarding how the application of microbial agents, alone or in combination, affects changes in the rhizosphere microbiome and its association with the bioactive components of medicinal materials. Methods: In this study, Epimedium pubescens Maxim. was employed as a model plant to examine the effects of 2 microbial agents ( Paenibacillus mucilaginosus and Bacillus subtilis ) applied individually and in combination on plant growth and the accumulation of bioactive components. Additionally, this study explored the relationship between the rhizosphere microbiome and plant development. Results: The application of microbial agents increased the yield of E. pubescens leaves by 20.30% to 33.66% and enhanced the total flavonol glycosides content by 11.40% to 29.94%. Meanwhile, microbial treatments reshaped the rhizosphere microbiome, promoted the enrichment of beneficial microorganisms (e.g., Frankia and Paenibacillus ), suppressed phytopathogenic fungi such as Didymella and Scytalidium , and enhanced the stability of the soil microbial co-occurrence network. The partial least squares path model suggested that microbial a...