Effects of butyric acid on the inhibition of Vibrio parahaemolyticus and its impacts on growth performance, intestinal microbiota, and water microbiota in Litopenaeus vannamei

作者：Ming Yin, Zhangyi Zheng, Rui Xing, Lefei Jiao, Moussa Gouife, Tinghong Ming, Fei Kong, Shuang Qiu, Jiajie Xu · 发表于：Aquaculture Reports · 年份：2025 · DOI：10.1016/j.aqrep.2025.103078 · 被引用次数：5 · 研究领域：Aquaculture disease management and microbiota、Vibrio bacteria research studies、Aquaculture Nutrition and Growth

Pathogenic Vibrio species have long posed a major challenge in shrimp aquaculture, while traditional antibiotic treatments are associated with numerous limitations, creating an urgent demand for novel preventive and therapeutic approaches. The present study employed in vivo and in vitro experiments to comprehensively evaluate the feasibility of butyric acid supplementation in Litopenaeus vannamei culture. In vitro antimicrobial assays demonstrated that under acidic conditions (pH 6), the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of butyric acid against V. parahaemolyticus were 20 mM and 40 mM, respectively. In contrast, under neutral conditions (pH 7), the MIC and MBC increased to 320 mM and 640 mM. At both MIC and MBC concentrations, butyric acid completely suppressed bacterial growth and significantly disrupted biofilm formation and cellular integrity. In vivo shrimp feeding trials revealed that dietary supplementation with 0.5 and 2.0 g/kg butyric acid significantly enhanced percent weight gain (PWG) and specific growth rate (SGR) ( P < 0.05). 0.5 g/kg butyric acid supplementation could reduce the feed conversion ratio ( P < 0.05). The addition of sodium butyrate (in its sodium salt form) significantly reduced the abundance of intestinal Vibrio, decreasing from 17.45 in the CK group to 8.63 %(SB0.5), 5.54 %(SB2.0), and 0.62 %(SB5.0) in the respective treatment groups, while promoting the proliferation of beneficial bacteria, partic...