Synergistic interaction in mixed pant growth promoting rhizobacteria consortium enhances biofilm formation and rhizosphere colonization to promote tomato (Solanum lycopersicum) growth

作者：Dandan Wang, Nan Zeng, Jiahe Pang, Chunji Li, Dingcun Zhang, Junliang Ge, Die Zhao, Jinmei Li, Ruoyan Ran, Xiangyu Gao, Rutao Gai, Ziqi Cao, Xinyue Bi, Ning Zhang, Bingxue Li · 发表于：Scientia Horticulturae · 年份：2025 · DOI：10.1016/j.scienta.2025.114383 · 被引用次数：11 · 研究领域：Plant-Microbe Interactions and Immunity、Mycorrhizal Fungi and Plant Interactions、Composting and Vermicomposting Techniques

While plant growth-promoting rhizobacteria (PGPR) consortia show enhanced benefits over single strains, the systematic design of functionally complementary synthetic communities and their synergistic mechanisms through biofilm-mediated root colonization remain poorly explored. This study aimed to screen and construct functionally complementary PGPR consortia, systematically evaluate their synergistic effects on biofilm formation in the tomato rhizosphere, and elucidate the underlying mechanisms regulating plant growth through enhanced root colonization and metabolic interactions. Three PGPR strains — Pantoea ananatis D1–28, Bacillus aryabhattai LAD, and Burkholderia cepacia 4–5—were selected for individual and combinatorial inoculation experiments. The results demonstrated that the D1–28/LAD combination exhibited significant synergistic advantages in both biofilm formation and plant growth promotion. This consortium markedly enhanced rhizosphere colonization and the synthesis of growth-promoting factors (such as N, P, siderophores, and IAA), thereby significantly improving tomato growth and up-regulating auxin biosynthesis and nitrogen transporter gene expression in roots. At an inoculum concentration of 10 6 cfu·mL −1 , the fresh and dry weights of tomato shoots increased by 186.40 % and 278.57 %, respectively, while root fresh and dry weights increased by 327.62 % and 543.68 %. Plant height, root length, root surface area, and root volume increased by 117.19 %, 207.29 %, 53...