Progress in serology and molecular biology of equine parasite diagnosis: sustainable control strategies

作者：Tengyu Wang, Xindi Chen, Yan Xu, Ya Su, Wa Gao, Chunxia Liu, Wenlong Wang · 发表于：Frontiers in Veterinary Science · 年份：2025 · DOI：10.3389/fvets.2025.1663577 · 被引用次数：2 · 研究领域：Helminth infection and control、Coccidia and coccidiosis research、Plant and fungal interactions

Internal parasitic infections are a persistent challenge for horse owners, in the absence of effective vaccines and the growing challenge of drug resistance, leading many researchers to view current control strategies as unsustainable. Despite slow progress over the past two decades, effective parasitic diagnosis remains crucial for controlling infections and preventing the growing issue of drug resistance. This review examines the research progress in serological and molecular biological diagnostic methods for major equine parasites. Currently, most diagnostic techniques are based on genes such as ITS1, ITS2, COI, and IGS, which have been applied to equine strongylids, including Strongylus spp., Cylicocyclus spp., and Cylicostephanus spp. These methods are particularly suitable for large-scale epidemiological studies and rapid species identification. Although many diagnostic methods have been developed, most remain confined to laboratory research and have seldom been used for real-time field diagnostics. Future research should prioritize precise diagnostic methods and clinically applicable alternatives. Additionally, whole genome sequencing has been widely used in eukaryotes for population genetics and the development of diagnostic markers. However, comprehensive genomic data on parasitic species infecting equines is still limited. With the decrease in sequencing costs in the post-genomic era, a growing number of genome assemblies are expected to be released soon. These geno...