Tetrahedral DNA Framework-Engineered Magnetic Nanozyme for Visual Biomarker Sensing via Alkaline Phosphatase-Triggered Signal Switching

作者：Mengde Zhao, Jiahui Zhang, Lihua Wang, Na Lü · 发表于：Analytical Chemistry · 年份：2025 · DOI：10.1021/acs.analchem.5c02300 · 被引用次数：10 · 研究领域：Advanced Nanomaterials in Catalysis、Advanced biosensing and bioanalysis techniques、Nanocluster Synthesis and Applications

Point-of-care (POC) detection of prostate-specific antigen (PSA) is critical for the early screening and monitoring of prostate cancer (PCa), which facilitates timely intervention and personalized treatment. However, existing POC platforms suffer from inadequate detection sensitivities, susceptibility to matrix interference, and complex sample pretreatment. To address these issues, we proposed a naked-eye and colorimetric sensing platform based on magnetic nanozyme (Fe 3 O 4 @ZIF-67@Pt) integrated with a tetrahedral DNA framework (TDF) and alkaline phosphatase (ALP)-triggered hydrolysis reaction for PSA detection with superior sensing performances. The as-prepared Fe 3 O 4 @ZIF-67@Pt nanocomposite synergistically integrates magnetic separation, DNA-conjugated interface engineering, and significantly enhanced peroxidase-like activity, thereby laying the foundation for the construction of high-performance colorimetric biosensors. TDF serves dual functions as a programmable biosensing element, including three vertex anchor ALP reporters for catalytic signal amplification, while the remaining vertex is hybridized with a PSA-specific aptamer to drive competitive target recognition. With the addition of PSA, it preferentially bound to the aptamer, creating a competitive reaction and leading to the release of TDF conjugated to the surface of the magnetic nanozyme. When the ALP-mediated hydrolysis reaction was introduced, the released TDF limited the generation of ascorbic acid (AA) ...