Developmental programming: Differing impact of prenatal testosterone and prenatal bisphenol-A -treatment on hepatic methylome in female sheep

作者：John Dou, Soundara Viveka Thangaraj, Yiran Zhou, Vasantha Padmanabhan, Kelly M. Bakulski · 发表于：Molecular and Cellular Endocrinology · 年份：2025 · DOI：10.1016/j.mce.2025.112655 · 被引用次数：3 · 研究领域：Effects and risks of endocrine disrupting chemicals、Birth, Development, and Health、Agriculture Sustainability and Environmental Impact

and a magnitude of difference of at least 5 % methylation between groups were considered differentially methylated. Results demonstrated substantial differential methylation in the liver tissues due to both treatments, with prenatal-T causing unique epigenetic modifications distinct from those induced by prenatal-BPA. Specifically, prenatal-T treatment resulted in 53 differentially methylated regions (DMRs), of which 31 were located in gene regions, including exons. Prenatal-BPA exposure led to 32 DMRs, with 22 associated with gene regions. These modifications were associated with genes governing lipid and glucose metabolism, potentially underlying the observed metabolic disruptions such as insulin resistance and dyslipidemia. Pathway analysis revealed that genes differentially methylated due to prenatal-T were involved in cellular organization, while those affected by prenatal-BPA were enriched in signal regulation pathways. The findings underscore how prenatal exposure to steroid excess and steroid-mimics influence epigenetic landscapes, contributing to metabolic disease programming.