Phytochemical Screening and Antidiabetic Potential of Paeonia emodi in Alloxan‐Induced Diabetic Rats: Investigative Study Toward Possible Mechanism

作者：Jirui Fan, Mohammad Ibrar, Mir Azam Khan, Abdullah, Muhammad Saeed Jan, Madeeha Shabnam, Maqsood Ur Rehman · 发表于：Food Science & Nutrition · 年份：2025 · DOI：10.1002/fsn3.70870 · 被引用次数：4 · 研究领域：Natural Antidiabetic Agents Studies、Bioactive natural compounds、Natural product bioactivities and synthesis

ABSTRACT Paeonia emodi has long been used in folk medicine to treat a variety of ailments, including diabetes, skin disorders, dropsy, cuts, wounds, ulcers, fever, and blood disorders, etc., which are generally categorized under the complications of diabetes mellitus. Various species of this genus have also been verified to possess strong anti‐diabetic activity. In this context, the current study was designed to investigate the antidiabetic potential to confirm the purported traditional use of P. emodi . Different solvent fractions of P. emodi , i.e., crude methanolic extract (Pe.Cr), n‐hexane (Pe.Hex), chloroform (Pe.Chf), ethyl acetate (Pe.EtAc), butanol (Pe.Bt) and aqueous (Pe.Aq) were used for in vitro studies against α‐glucosidase, α‐ amylase, dipeptidyl peptidase‐4 (DPP‐4), and protein tyrosine phosphatase 1B (PTP‐1B) using a spectrophotometer and microplate reader. Among all fractions, Pe.Bt had the most prominent activity and was subjected to GC–MS analysis, in vivo anti‐diabetic, pancreas protective, and hepatoprotective studies in alloxan‐induced diabetes rats. The animals administered with alloxan (except group1) having FBGL higher than 220 mg/dL were selected and placed in different groups. The first group, which served as a normal control, received normal saline. The second group received a 5% tween‐80 suspension, which served as diabetic control. The 3rd, 4th and 5th groups were administered Pe.Bt at the doses of 150, 300, and 500 mg/kg body weight, respectively...