Genome-replicating HC-AdV: A novel high-capacity adenoviral vector class featuring enhanced in situ payload expression

作者：Jonas Kolibius, Fabian Weiss, Patrick C. Freitag, Andreas Plückthun · 发表于：Molecular Therapy — Methods & Clinical Development · 年份：2025 · DOI：10.1016/j.omtm.2025.101582 · 被引用次数：3 · 研究领域：Virus-based gene therapy research、Viral Infectious Diseases and Gene Expression in Insects、CAR-T cell therapy research

-replication of the delivered HC-AdV genome and up to 20-fold increased reporter fluorescence. Our novel vector retained a large transgene capacity (22 kb) and, unlike RC-AdVs, did not induce a cytopathic effect or host cell killing. Together, these data describe a novel delivery platform potentially allowing more efficacious vaccination and vector-mediated therapies.