Ganglioside Profiling Uncovers Distinct Patterns in High-Risk Neuroblastoma

作者：Claudia Paret, Arthur Wingerter, Larissa Seidmann, Arsenij Ustjanzew, Shobha Sathyamurthy, Jannis Ludwig, Philipp Schwickerath, Chiara Brignole, Fabio Pastorino, Saskia Wagner, Khalifa El Malki, Wilfried Roth, Roger Sandhoff, Joerg Faber · 发表于：International Journal of Molecular Sciences · 年份：2025 · DOI：10.3390/ijms26178431 · 被引用次数：5 · 研究领域：Neuroblastoma Research and Treatments、Erythrocyte Function and Pathophysiology、Cancer, Hypoxia, and Metabolism

High-risk (HR) neuroblastoma (NBL) patients often receive standardized treatment despite wide variations in clinical outcomes, underscoring the need for improved stratification tools. A distinguishing feature of NBL is the patient-specific expression of gangliosides (GGs), particularly GD2, which may serve as biomarkers. We analyzed GG profiles in 18 patient-derived tumors and 11 NBL cell lines using thin-layer chromatography and mass spectrometry. Expression of 0-, a-, and b-series GGs was examined and correlated with clinical risk, outcome, and gene expression data. Low-risk (LR) tumors expressed higher levels of complex b-series GGs. In HR tumors, five GG profiles (A–E) were identified. Profile A featured complex b-series GGs; B showed GD2 dominance; C showed synthesis arrest at GM3 or GD3 due to low expression of the GM2/GD2 synthase, encoded by the B4GALNT1 gene; D included complex a- and b-series GGs; and E was marked by GM2 and GD1a prevalence. B4GALNT1 expression served as a prognostic marker. Relapsed tumors following anti-GD2 therapy typically exhibited reduced GD2 levels, except for one profile A tumor that displayed a ceramide anchor shorter than those found in LR tumors. Astonishingly, the ceramide anchor composition of GD2 itself appears to separate LR and HR NBL, hinting at a role of ceramide synthases in NBL biology. All cell lines expressed GM2, but exhibited very low levels of complex b-series GGs. Profile C was found only in cell lines of the mesenchymal su...