GC-MS profiling, in vitro and in silico antibacterial and antioxidant potential of crude secondary metabolites of an endophytic bacterium isolated from Enhydra fluctuans lour

作者：Abdul Ali Bhuiyan, Mourad A. M. Aboul‐Soud, Md. Nazim Uddin, Kishor Mazumder, A. U. Akash, Reem M. Aljowaie, John P. Giesy, Mohit Agrawal, Shariful Haque · 发表于：Scientific Reports · 年份：2025 · DOI：10.1038/s41598-025-17314-5 · 被引用次数：12 · 研究领域：Microbial Natural Products and Biosynthesis、Essential Oils and Antimicrobial Activity、Phytochemistry and Bioactivity Studies

This study investigates the bioactive potential of secondary metabolites produced by endophytic bacteria isolated from Enhydra fluctuans Lour., a medicinal plant known for its antioxidant and antimicrobial properties. Fresh leaves were collected, surface-sterilized, and used to isolate bacterial endophytes. The isolates were identified morphologically and confirmed by 16S rRNA gene sequencing. Metabolites were extracted using ethyl acetate and assessed for antibacterial activity via the disc diffusion method and antioxidant capacity by DPPH radical scavenging assay. GC-MS was used to identify volatile bioactive substances. The isolate showed 91.75% similarity with Pseudomonas campi (NR_181172.1), indicating a distant relationship and suggesting it may represent a novel or uncharacterized taxon. The isolate was mostly sensitive to Ciprofloxacin (5 µg). Its ethyl acetate crude extract showed moderate antibacterial activity against Staphylococcus aureus with a 13.4 ± 0.21 mm inhibition zone at 500 µg/disc, compared to kanamycin (30 µg/disc). Antioxidant activity showed an IC 50 of 61.66 ± 0.33 µg/mL, versus 17.38 ± 0.63 µg/mL for ascorbic acid. GC-MS identified 19 bioactive compounds, with Phenol, 3,5-bis(1,1-dimethylethyl)-, 1,9-Diazaspiro (4,4 )nonane-2,8-dione, and Octacosanol as major constituents. In silico analyses showed that 9,10-anthracenedione, 2-[(tert-butylamino)methylcarbamoyl] exhibited strong antibacterial and antioxidant potential, with high binding affinities to...