Oral Pathobionts Aggravate Myocardial Infarction Through Mobilization of B2 Cells

作者：Bo‐Yan Chen, Hong Zhu, Yulin Li, Jun Zhang, Shuo Xu, Lu-Jun Zhou, Lin‐Juan Du, Ting Liu, Xue‐Nan Sun, Guocai Tian, Jie Li, Xiao-Qian Meng, Huilin Ye, Xuebing Bai, Wen-Zhen Lin, Huxiao Li, Jianxuan Li, Jianyong Sun, Yan Liu, Wuchang Zhang, Pengfei Zhang, Jing-Yuan Fang, Daqian Xu, Mengfei Yu, Yuxiang Dai, Qianming Chen, Sheng‐Zhong Duan · 发表于：Circulation · 年份：2025 · DOI：10.1161/circulationaha.125.074837 · 被引用次数：15 · 研究领域：Oral microbiology and periodontitis research、Atherosclerosis and Cardiovascular Diseases、Neutrophil, Myeloperoxidase and Oxidative Mechanisms

BACKGROUND: Myocardial infarction (MI) is a high-prevalence disease that threatens human survival and quality of life worldwide. Considerable evidence has suggested that periodontitis (PD) is detrimental to MI. However, the direct impact of PD on MI is unclear; which oral pathobionts contribute to and how microbial signals regulate the pathogenesis of MI remain obscure. METHODS: The effect of PD on MI was assessed in a mouse model that combined ligature-induced PD with MI. Ectopic accumulation of oral pathobionts in infarcted hearts was identified by bacterial sequencing and fluorescence in situ hybridization. Oral pathobionts–reactive B2 cells detrimental for MI were determined by flow cytometry and verified in gnotobiotic mice. Several mouse strains, including CD45.1, Kaede, and knockout strains for Ighm, Rag1, C-X-C motif chemokine ligand 13, S1pr, interleukin-6, and tumor necrosis factor-α, were used for mechanistical exploration. Mutant strains of Fusobacterium nucleatum and Porphyromonas gingivalis deficient in adhesion proteins were generated to investigate bacterial mechanism involved in MI exacerbation. Patients with MI with or without PD were recruited to test the deteriorating impact of PD on MI and the association between oral pathobionts and cardiac function. RESULTS: Ligature-induced PD and subgingival plaques of patients with PD exacerbated MI in mice by oral pathobionts. Prevotella intermedia , Fusobacterium nucleatum , Selenomonas sputigena , Porphyromonas gi...