Phosphorus absorption by mycorrhizal extraradical hyphae accelerates plant growth and active ingredient production in Polygonum cuspidatum

作者：Xin-Ping An, Ze-Zhi Zhang, Li-Jun Zhou, Hai-Dong Feng, Rui-Ting Sun, Abeer Hashem, Elsayed Fathi Abd-Allah, Qiang‐Sheng Wu · 发表于：Chemical and Biological Technologies in Agriculture · 年份：2025 · DOI：10.1186/s40538-025-00844-5 · 被引用次数：8 · 研究领域：Mycorrhizal Fungi and Plant Interactions、Fungal Biology and Applications、Legume Nitrogen Fixing Symbiosis

Arbuscular mycorrhizal (AM) fungi have demonstrated the capacity to increase active ingredient concentrations in industrial plants such as Polygonum cuspidatum, whereas it is still uncertain whether this effect is mediated by AM-facilitated nutritional absorption like phosphorus (P). In this study, a microsystem was utilized to deliver extra P to the hyphal chamber, thereby examining the impacts of hyphal P absorption on plant growth, P acquisition, phosphatase concentration, bioactive compound concentrations, and the expression of resveratrol-related genes in P. cuspidatum. The supplementation of P led to significant increases in Funneliformis mosseae colonization rates in roots and hyphal lengths in soil. AM inoculation significantly improved plant height (34.3–84.7%) and root biomass (10.9–27.8%), particularly when P was supplied to the hyphal chamber. Compared with the uninoculated treatment, AM inoculation also distinctly elevated leaf and root P levels by 110.9–252.2% and 33.0–179.5%, respectively, aligning with an increase in soil acid, neutral, and alkaline phosphatase concentrations. Roots served as the primary site for resveratrol biosynthesis. Inoculation with F. mosseae increased bioactive compound concentrations in both leaves and roots to varying degrees, with the observed increase being greater when hyphae could pass through a 37 μm mesh (permitting hyphal access) into the P-supplemented hyphal chamber, as opposed to a 0.45 μm mesh (restricting hyphal access). ...