3D bioprinted nanozyme-enhanced GelMA hydrogel with antioxidant/anti-inflammatory potential for bone repair

作者：Yuting Chen, Huixiu Han, Lei Liu, Junxu Yang, Hao Wang, Ying Tan, Feiying Yin, Jianwen Cheng, Zheng Li, Jinmin Zhao · 发表于：Nano Research · 年份：2025 · DOI：10.26599/nr.2025.94907979 · 被引用次数：2 · 研究领域：Advanced Nanomaterials in Catalysis、Nanoplatforms for cancer theranostics、Bone Tissue Engineering Materials

The repair of large-scale bone defects is still a challenge in clinical orthopedics. Especially, Excessive reactive oxygen species (ROS)-induced oxidative stress injury greatly affected bone healing. In this study, we innovatively developed an antioxidant 3D-bioprinted M-Mn 3 O 4 @Gel by integrating M-Mn 3 O 4 nanozyme into photo-crosslinked gelatin methacryloyl (GelMA) for the therapy of bone defects. Results showed that the incorporation of M-Mn 3 O 4 not only enhanced the mechanical properties of the nanocomposite hydrogel with the compressive modulus 141.79% higher than that of pure GelMA, but also maintained excellent 3D printability. In vitro studies confirmed that the 3D-printed M-Mn 3 O 4 @Gel exhibited favorable biocompatibility and cell adhesion. It significantly reduced oxidative stress through efficient ROS scavenging, restored mitochondrial function, and ultimately demonstrated remarkable osteogenic capacity, highlighting the efficacy of control-released nanozymes. More importantly, under near-infrared (NIR) irradiation, M-Mn 3 O 4 @Gel demonstrated further enhanced ROS-scavenging capacity and bone regeneration potential. Mechanistically, M-Mn 3 O 4 @Gel promoted osteogenesis by upregulating heat shock protein 40kDa (HSP40) and HSP70 expression, effectively mitigating the overactivation of the Nrf2 pathway. This study innovatively combines nanozyme technology with 3D-printed hydrogel materials, offering a novel strategy to address the challenge of oxidative s...