OSMAC-Driven Discovery of Six New Alkaloids from the Cold-Seep-Derived Fungus Talaromyces amestolkiae HDN21-0307

作者：Xinsheng Huang, Jiajin Wu, Luning Zhou, Zhengjie Wang, Qian Che, L.L. Chen, Wenxue Wang, Tianjiao Zhu, Dehai Li · 发表于：Marine Drugs · 年份：2025 · DOI：10.3390/md23090337 · 被引用次数：4 · 研究领域：Microbial Natural Products and Biosynthesis、Marine Sponges and Natural Products、Genomics and Phylogenetic Studies

Six new alkaloid compounds, including two rare aromatic nitrile compounds talaronitriles A–B (1–2), a novel oxime-functionalized azadiphilone analogue talarooxime A (3), a new phenylhydrazone alkaloid talarohydrazone E (4), and two new dipeptide compounds talarodipeptides A–B (5–6), were isolated from the deep-sea cold-seep-derived fungus Talaromyces amestolkiae HDN21-0307 via OSMAC approach. Compound 1 is the first natural naphthalene compound with cyano groups. Compound 3 represents the first natural product containing an oxime-functionalized azadiphilone scaffold. Their structures and absolute configurations were elucidated through spectroscopic data analysis and quantum chemical calculations. Notably, compound 3 demonstrated moderate DPPH free-radical-scavenging activity, with an IC50 value of 29.41 μM.