Identification of a novel aminoglycoside nucleotidyltransferase ANT(3″)-Ic from Citrobacter telavivum S24

作者：Junwan Lu, Mei Zhu, Jingzeng Ye, Leyao Xu, Chunhan Song, Kewei Li, Qiyu Bao, Xi Lin · 发表于：BMC Microbiology · 年份：2025 · DOI：10.1186/s12866-025-04282-z · 被引用次数：1 · 研究领域：Carbohydrate Chemistry and Synthesis、Biochemical and Molecular Research、Glycosylation and Glycoproteins Research

Understanding the antibiotic resistance profile of the emerging pathogen Citrobacter telavivum S24 is important for clinical care, surveillance, and research. C. telavivum S24, a strain that exhibits resistance to both streptomycin and spectinomycin, was isolated from the soil at a broiler chicken farm. The strain was identified by whole-genome sequencing and average nucleotide identity analyses. Annotation of the resistance genes revealed that a novel aminoglycoside resistance gene, designated ant(3″)-Ic , is located on the chromosome of C. telavivum S24. This gene exhibits 52% similarity with the previously functionally characterized resistance gene aadA23 . It distinguishes itself from ant(3″)-Ia and ant(3″)-Ib genes, and represents an independent branch. The ant(3″)-Ic gene is recognized as an inherent resistance gene of C. telavivum within a conserved genetic environment. The product of the ant(3″)-Ic gene is the aminoglycoside nucleotidyltransferase ANT(3″)-Ic, which has a theoretical pI of 5.05 and a molecular mass of 29 kDa. ANT(3”)-Ic inactivates streptomycin and spectinomycin with the K m of 46.13 and 115.90 µM, respectively. The discovery of the novel antibiotic resistance gene ant(3″)-Ic and the functional characterization of ANT(3”)-Ic will facilitate more targeted surveillance and treatment strategies for preventing and controlling zoonotic pathogens of zoonotic diseases.