Ultrasensitive optoelectronic biosensor arrays based on twisted bilayer graphene superlattice

作者：Bowen Du, Xilin Tian, Zhi Chen, Yanqi Ge, Chuanghu Chen, Haiyan Gao, Zhongyang Liu, Jung‐Chen Tung, Dror Fixler, Songrui Wei, Shi Chen, Han Zhang · 发表于：National Science Review · 年份：2025 · DOI：10.1093/nsr/nwaf357 · 被引用次数：137 · 研究领域：Graphene research and applications、Advanced biosensing and bioanalysis techniques、2D Materials and Applications

Recent advances in twistronics have revealed tunable optoelectronic properties in twisted bilayer graphene (tBLG), including angle-dependent dielectric responses and enhanced light absorption due to van Hove singularity (VHS). However, achieving high photoresponsivity in tBLG-based sensors typically requires intense illumination. We present an ultrasensitive optoelectronic biosensor integrating tBLG superlattices with Au nanodisks and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a via DNA origami. By aligning the 9.4° tBLG's VHS absorption spectrum with Au nanodisks' plasmonic resonance at 60 μW, we achieve a 7-fold photocurrent enhancement over pristine tBLG. CRISPR-Cas12a-mediated trans-cleavage dynamically modulates the local dielectric environment, enabling sub-femtomolar (44.63 attomolar, aM) nucleic acid detection without external amplification. Clinical validation using lung cancer samples shows high concordance with quantitative polymerase chain reaction (qPCR), demonstrating real-time, label-free detection of microRNA (miRNA). This hybrid platform combines moiré-engineered optoelectronics with programmable bio-nanoarrays, offering a scalable solution for precision diagnostics with ultralow detection limits and rapid response times.