A Cell-Surface-Targeted Fluorogenic Probe for Detection of Sulfatase 2 Activity

作者：Yi Ding, Toshiyuki Kowada, Toshitaka Matsui, Norihiko Sasaki, Shin Mizukami · 发表于：Bioconjugate Chemistry · 年份：2025 · DOI：10.1021/acs.bioconjchem.5c00251 · 被引用次数：2 · 研究领域：Lipid Membrane Structure and Behavior、Molecular Sensors and Ion Detection、Neonatal Respiratory Health Research

High Resolution Image Download MS PowerPoint Slide Sulfatase 2 (Sulf-2), an extracellular sulfatase that modulates the sulfation pattern of heparan sulfate proteoglycans (HSPGs), serves as a critical biomarker for various pathological conditions. Monitoring Sulf-2 activity in living cell samples provides valuable insights for diagnostic applications and therapeutic evaluation of Sulf-2-related diseases. In this study, we developed a novel plasma-membrane-targeted fluorogenic probe, MAR-S, to visualize the activity of Sulf-2 secreted by living cells. Upon incubation with Sulf-2-containing culture supernatant from pancreatic cancer cells, MAR-S exhibited a significant increase in fluorescence at approximately 540 nm. Notably, MAR-S allowed for time-lapse monitoring of endogenous Sulf-2 activity in living cancer cells overexpressing Sulf-2, demonstrating its potential as a valuable tool for Sulf-2-related cancer diagnostics and therapeutic research.