Biocontrol of the zearalenone‐producer Fusarium ipomoeae on soybean pods using an avirulent Lasiodiplodia theobromae isolate

作者：Lihua Tang, Xin Chen, Yong‐hui Jiang, Jia‐Jia Cao, Ting Wu, Xingchen Wang, Yang Shen, X. P. Zhang, Siyuan Liu, Suyan Wang, Pedro Laborda · 发表于：Pest Management Science · 年份：2025 · DOI：10.1002/ps.70150 · 被引用次数：2 · 研究领域：Mycotoxins in Agriculture and Food、Fungal Plant Pathogen Control、Plant Disease Resistance and Genetics

Abstract BACKGROUND Fusarium pod blight is emerging as a destructive disease in soybean‐producing regions. Currently, strategies for the management of Fusarium pod blight are lacking. This study aimed to examine the biocontrol activity of a new avirulent Lasiodiplodia theobromae isolate for the management of Fusarium pod blight. RESULTS The avirulent isolate L. theobromae NJC90 was obtained from Fusarium ipomoeae ‐infected soybean pods and identified via morphological analysis and gene sequencing. Lasiodiplodia theobromae NJC90 showed high sporulation capacity and rapid growth, and could colonize soybean pods, maintaining its population for 12 days. Lasiodiplodia theobromae NJC90 competed for space with F. ipomoeae in soybean pods, reduced F. ipomoeae spore germination between 20.4% and 43.7% via nutrient competition with the pathogen, and inhibited zearalenone biosynthesis in vitro by 57.0%. Preventive and curative applications of 1 × 10 8 spores/mL L. theobromae NJC90 reduced F. ipomoeae ‐caused lesions by 71.5% and 24.4%, respectively. Interestingly, preventive and curative applications of 1 × 10 8 spores/mL L. theobromae NJC90 reduced zearalenone levels in soybean pods by 100% and 58.3%, respectively, achieving concentrations under the maximum level established by the European Union Commission. CONCLUSION The use of L. theobromae as a biocontrol agent was studied for the first time, revealing the first biocontrol approach for the management of Fusarium pod blight. This is...