Evaluation of Pepsinogen I, II, Gastrin 17 and Helicobacter pylori IgG in Atrophic Gastritis: A Head‐To‐Head Comparison of Lateral Flow and Enzyme‐Linked Immunosorbent Assays

作者：Luochengling Xiang, Ying Zhou, Xiaopei Guo, Michiel C. Mommersteeg, Stella A. V. Nieuwenburg, Maikel P. Peppelenbosch, Manon C.W. Spaander, Gwenny M. Fuhler · 发表于：Helicobacter · 年份：2025 · DOI：10.1111/hel.70066 · 被引用次数：3 · 研究领域：Helicobacter pylori-related gastroenterology studies、Gastrointestinal motility and disorders、Inflammatory Bowel Disease

BACKGROUND: A lateral flow assay (LFA) incorporating several biomarkers, including pepsinogen I (PGI), pepsinogen II (PGII), Gastrin-17 (G-17), and Helicobacter pylori IgG, enables the rapid non-invasive detection of atrophic gastritis (AG). However, its diagnostic performance compared to conventional enzyme-linked immunosorbent assay (ELISA) has not been established. METHODS: This head-to-head comparison study included participants from a prospective and multicenter cohort. Patients with gastric premalignant lesions underwent endoscopy, and fasting serum samples were collected for biomarker analysis using both LFA and ELISA. RESULTS: A total of 204 patients were included in this study. LFA demonstrated diagnostic specificity for AG comparable to ELISA, with specificity rates of 95.74% (95% CI [85.75%-99.24%]) for LFA and 100.00% (95% CI [92.44%-100.00%]) for ELISA (p = 0.49). Both methods showed similar performance in detecting H. pylori infection, with an AUC of 0.754 (95% CI [0.616-0.891]) for LFA and 0.778 (95% CI [0.633-0.922]) for ELISA (p = 0.70). For identifying autoimmune gastritis in corpus AG, a reduced PGI/PGII ratio combined with elevated G-17 levels provided excellent discrimination, achieving an AUC of 0.926 (95% CI [0.870-0.926]) for LFA and 0.924 (95% CI [0.861-0.924]) for ELISA. CONCLUSION: The LFA assay is a feasible, rapid, and non-invasive tool for assessing gastric functional mucosa. Its diagnostic performance for detecting AG is comparable to ELISA, mak...