High Sensitivity and Selectivity Electrochemiluminescence Sensor Based on the Regulation of Wettability in the Microchannel for the Detection of Glutathione in the Rat Brain

作者：Huabin Cai, Peng Wu, Yanling Huang, Jian Shou, Fang Luo, Zhenyu Lin, Minyi Yin · 发表于：Analytical Chemistry · 年份：2025 · DOI：10.1021/acs.analchem.5c02793 · 被引用次数：3 · 研究领域：Advanced biosensing and bioanalysis techniques、Nanoplatforms for cancer theranostics、Sulfur Compounds in Biology

Detection of glutathione (GSH) in the brain provides critical support for investigating the role of GSH in regulating brain function and the mechanism of related diseases. Regulation of the current going through the microchannel can regulate the electrochemiluminescence (ECL) response of the microchannel-based ECL system. In this study, an ECL sensor based on the regulation of wettability of the microchannel was constructed for the first time and successfully applied to detect GSH in the rat brain. The hydrophobic compound bis(triethoxysilylpropyl)disulfide (BTSD) was modified inside the microchannel, and the wettability of the microchannel interface was regulated by the reduction of disulfide bonds in BTSD by GSH, which in turn altered the ionic current and ECL signal of the microchannel-based ECL system. Under optimal conditions, the sensor showed good detection of GSH in the range of 100 nM-10 mM, with a detection limit of 22.4 nM. The sensor has good selectivity and is also reusable. It has been applied to detect GSH in the rat hippocampus with satisfied results.