Dual DNAzyme-MoS2/GDY Catalytic Assembly Enables Smartphone-Based Multiplex Detection of Sugarcane Pokkah Boeng Pathogens at Sub-Femtomolar Levels

作者：Bao‐Quan Fu, Rongshuai Che, Zeping Wang, Defen Feng, Jun Yan, Ke‐Jing Huang, Yu Ya, Xuecai Tan · 发表于：Analytical Chemistry · 年份：2025 · DOI：10.1021/acs.analchem.5c02478 · 被引用次数：6 · 研究领域：Advanced biosensing and bioanalysis techniques、Studies on Chitinases and Chitosanases、Biosensors and Analytical Detection

Rapid on-site detection of sugarcane pokkah boeng disease caused by Fusarium pathogens remains challenging due to the lack of portable platforms combining high sensitivity and multiplexing capability. Here, we present a self-powered biosensor integrating a dual DNAzyme-driven catalytic system with a MoS 2 /graphdiyne (GDY) nanohybrid-modified biofuel cell (EBFC) for simultaneous detection of Fusarium sacchari and Fusarium verticillioides . The key innovation lies in the windmill-shaped dual DNAzyme structure that enables Mn 2+ /Mg 2+ -dependent target recycling, synergistically coupled with the hybridization chain reaction (HCR) and triplex catalytic hairpin assembly (TCHA) for exponential signal amplification. The MoS 2 /GDY nanohybrid provides an ideal conductive substrate with 3.8-fold higher DNA loading capacity than pristine MoS 2, while the integration of a charge-storage capacitor boosts detection sensitivity by 10.4- and 9.8-fold compared with conventional EBFCs through transient current amplification. The smartphone-coupled system achieves unprecedented detection limits of 21.3 aM ( F. sacchari ) and 54.3 aM ( F. verticillioides ) with a dynamic range spanning 5 orders of magnitude (0.1 fM-10 nM), demonstrating excellent specificity against non-target pathogens (more than 95% signal discrimination). This smartphone-integrated biosensor represents a field-ready diagnostic tool for rapid on-site screening of sugarcane fungal pathogens, offering a transformative approac...