Formula Screening and Optimization of Physical and Chemical Properties for Cultivating Flammulina filiformis Using Soybean Straw as Substrate

作者：Ruixiang Sun, Han Jiandong, Peng Yang, Shude Yang, Hongyan Xie, Jin Li, Chunyan Huang, Qiang Yao, Q. Wang, Mai–He Li, Xuerong Han, Zhiyuan Gong · 发表于：Horticulturae · 年份：2025 · DOI：10.3390/horticulturae11080947 · 被引用次数：4 · 研究领域：Fungal Biology and Applications、Biocrusts and Microbial Ecology、Algal biology and biofuel production

Recently, there has been a growing interest in using agricultural and forestry residues to cultivate Flammulina filiformis. However, there is limited research on cultivating F. filiformis with soybean straw as a substrate. This study systematically optimized the cultivation formula for F. filiformis using soybean straw as the raw substrate and explored the effects of the water content, carbon-to-nitrogen ratio (C/N ratio), substrate particle size, and substrate loading on its growth and development. By replacing corncob, wheat bran, and soybean hulls with soybean straw and increasing the proportion of rice bran, the cultivation formula for growing F. filiformis was optimized. We found that the maximum fruiting body yield of 405 g (330 g dry substrate per bottle) and a biological efficiency of 122.73% were achieved using a substrate mixture of 25% soybean straw, 20% corncob, 20% cottonseed hull, 25% rice bran, 8% wheat bran, 1% CaCO3, and 1% shellfish powder. The yield and biological efficiency of fruiting bodies cultivated on the substrate containing 25% soybean straw did not show significant differences compared to the control group. However, the cultivation formula containing 25% soybean straw yielded F. filiformis with significantly higher levels of amino acids, essential amino acids, and fat. These findings suggest that the 25% soybean straw substrate formulation can serve as a viable alternative to the control formulation for the cultivation of F. filiformis, although va...