3D cell-laden scaffold printed with brain acellular matrix bioink

作者：Aobo Zhang, Siyu Zhu, Boyu Sun, Chengrui Nan, Lulu Cong, Zongmao Zhao, Liqiang Liu · 发表于：Journal of Nanobiotechnology · 年份：2025 · DOI：10.1186/s12951-025-03644-z · 被引用次数：7 · 研究领域：3D Printing in Biomedical Research、Innovative Microfluidic and Catalytic Techniques Innovation、Pluripotent Stem Cells Research

BACKGROUND: Intracerebral hemorrhage (ICH) is a severe neurological disorder characterized by bleeding within the brain tissue, typically associated with factors such as hypertension, cerebrovascular disease, and trauma. The transplantation of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) has demonstrated promising effects in restoring neurological function in ICH rats; however, limited retention of these cells significantly impedes their efficacy. To address this limitation, we developed a bioink composed of decellularized extracellular matrix (dECM) and hUCMSCs, which was synthesized into 3D cell-laden scaffold through 3D bioprinting. This approach aims to extend the retention of hUCMSCs and create an early vascular microenvironment, thereby partially compensating for the drawbacks of hUCMSC transplantation and improving neurological function in ICH rats. METHODS: This study aimed to explore the use of a bioink formed by mixing 15% gelatin and 3% sodium alginate with a dECM solution, in conjunction with hUCMSCs, for 3D bioprinting of 3D cell-laden scaffold. The viscosity, morphology, and biocompatibility of the bioink were characterized using rheological analysis, scanning electron microscopy (SEM), and hematoxylin and eosin (HE) staining. Following printing, a live/dead assay kit was employed to assess the viability of hUCMSCs within the 3D cell-laden scaffold. ICH model rats were randomly assigned to four groups: (1) SHAM group; (2) ICH group; (3) ICH + 3D...