Precise mapping and candidate gene identification of the Rmd_JY47 locus conferring resistance to powdery mildew in soybean

作者：Yongsheng Sang, Yu‐Min Wang, Xiaodong Liu, Yue Liu, Min Wen, Lingchao Dong, Guangxun Qi, Cuiping Yuan, Jiahui Yang, Jian Zhang, Hongkun Zhao, Yingshan Dong · 发表于：Industrial Crops and Products · 年份：2025 · DOI：10.1016/j.indcrop.2025.121681 · 研究领域：Soybean genetics and cultivation、Plant pathogens and resistance mechanisms、Legume Nitrogen Fixing Symbiosis

Powdery mildew (PM), caused by Erysiphe diffusa , is a major constraint on soybean production worldwide. Host resistance is central to PM management, yet, the scarcity of durable resistance genes limits breeding process. In this study, a quantitative trait locus, Rmd_JY47 with a logarithm-of-odds score of 7.19 and a phenotypic contribution rate of 9.65 % was identified at the distal end of chromosome 16 (967.6-Kb) utilizing a dense genetic map constructed from 276 recombinant inbred lines originating from parental strains displaying resistance and susceptibility. Within this region, 85 candidate genes were identified, including 25 encoding LRR-RLP or RLKs and four RNA or DNA-binding proteins. Expression profiling after PM infection highlighted five genes— Glyma.16G167700 , Glyma.16G170900 , Glyma.16G171500 , Glyma.16G171700 , and Glyma.16G171800 —with significant differential expression and genetic variation between parental lines. These candidates likely function through LRR receptor-mediated immunity and post-transcriptional regulation, contributing to a layered defense response. The results provide valuable resources for marker-assisted selection and gene pyramiding, and offer insights into soybean– E. diffusa interactions to support molecular design breeding.