Phylogenetic and functional analysis of MYB genes unraveling its role involved in anthocyanin biosynthesis in H macrophylla

作者：Yingying Liao, Wenwen Zhao, Yu Wang, Sumeiqi Zhao, Shuo Gu, Chao Zhang, Jiqing Peng, Song Sheng, Sen Wang · 发表于：Scientific Reports · 年份：2025 · DOI：10.1038/s41598-025-14216-4 · 被引用次数：4 · 研究领域：Plant Gene Expression Analysis、Phytochemicals and Antioxidant Activities、Plant biochemistry and biosynthesis

Flower color serves as a pivotal ornamental trait in plants, significantly impacting their aesthetic value. H macrophylla is particularly esteemed for its large, vibrant flowers and the remarkable variability in color it exhibits. In this study, the MYB gene family within H. macrophylla was comprehensively examined, resulting in the identification of 72 MYB genes through genomic screening utilizing the Hidden Markov Model (HMM) method. A physicochemical analysis revealed a diverse range of properties among these proteins, with sizes varying from 65 to 770 amino acids; notably, six of these proteins were classified as stable. Predominantly, MYB proteins were localized within the nucleus, although some were also detected in other organelles, indicating a multifaceted role in cellular function. Phylogenetic analysis established that 13 MYB genes from H. macrophylla can be categorized into five distinct subfamilies based on the classifications observed in Arabidopsis thaliana, suggesting their involvement in the biosynthetic pathways of anthocyanins and proanthocyanidins. Expression analysis indicated a significant correlation between specific R2R3-MYB transcription factors, particularly HmMYB54, and the accumulation of anthocyanins during various flowering stages. qPCR results showed that the expression of HmMYB54 was consistent with the accumulation trend of 'Forever Summer' colors in hydrangea, while HmMYB61 was just the opposite, which was consistent with RNAseq, suggesting t...