Scalable and lightweight deep learning for efficient high accuracy single-molecule localization microscopy

作者：Yue Fei, Shuang Fu, Shi Wei, Ke Fang, Ruixiong Wang, Tianlun Zhang, Yiming Li · 发表于：Nature Communications · 年份：2025 · DOI：10.1038/s41467-025-62662-5 · 被引用次数：9 · 研究领域：Advanced Fluorescence Microscopy Techniques、Advanced Electron Microscopy Techniques and Applications、Force Microscopy Techniques and Applications

Deep learning has significantly improved the performance of single-molecule localization microscopy (SMLM), but many existing methods remain computationally intensive, limiting their applicability in high-throughput settings. To address these challenges, we present LiteLoc, a scalable analysis framework for high-throughput SMLM data analysis. LiteLoc employs a lightweight neural network architecture and integrates parallel processing across central processing unit (CPU) and graphics processing unit (GPU) resources to reduce latency and energy consumption without sacrificing localization accuracy. LiteLoc demonstrates substantial gains in processing speed and resource efficiency, making it an effective and scalable tool for routine SMLM workflows in biological research. This study presents LiteLoc, a lightweight and scalable AI model for efficient and accurate single molecule localization microscopy data analysis, bringing real-time deep-learning-based analysis to the era of high throughput super resolution imaging.