Structural characterization of a purified polysaccharide from Chrysanthemum vestitum and its effects on acute lung injury in mice

作者：Shujuan Xue, Ruixue Li, Jishu Wang, Xiaojun Li, Yuchen Guo, Yisen Qiao, Bao Zhang, Lili Wang, Suiqing Chen · 发表于：Chemical and Biological Technologies in Agriculture · 年份：2025 · DOI：10.1186/s40538-025-00822-x · 被引用次数：2 · 研究领域：Polysaccharides and Plant Cell Walls、Natural Compound Pharmacology Studies、Herbal Medicine Research Studies

Chrysanthemum vestitum, a perennial plant in the Compositae family and closely related to Chrysanthemum morifolium, has long been used as both food and medicine. To explore the structural characteristics and potential anti-inflammatory mechanisms of polysaccharides derived from C. vestitum, a homogeneous polysaccharide, CVP_C, was isolated from C. vestitum using hot water extraction and alcohol precipitation. The crude extract was then purified via DEAE-52 cellulose chromatography and gel filtration system. Structural characterization of CVP_C was performed by Fourier-transform infrared (FT-IR) spectroscopy, molecular weight determination, scanning electron microscopy (SEM), monosaccharide composition analysis, thermogravimetric analysis (TGA), nuclear magnetic resonance (NMR) spectroscopy, and methylation analysis. An LPS-induced ALI mouse model was established to assess the protective effects of CVP_C. Inflammatory cytokine levels (IL-6 and TNF-α) in lung tissues and bronchoalveolar lavage fluid (BALF) were quantified, and histopathological changes were evaluated. In addition, gut microbiota profiling was employed to study the underlying mechanisms. Structural analysis revealed that CVP_C is a xylan-type polysaccharide and has a molecular weight of 25.049 kDa. It is primarily composed of mannose (Man), galactose (Gal), glucosamine (GlcN), glucose (Glc), glucuronic acid (GlcA), xylose (Xyl), rhamnose (Rha), and arabinose (Ara). The backbone structure consisted of → 4)-β-D-Xy...