Ultrahigh-Throughput Multiplexed Screening of Purified Protein from Cell-Free Expression Using Droplet Microfluidics

作者：Anqi Chen, Wentao Xu, Xinge Zhang, Junming Lao, Xing Zhao, Karla Milčić, David A. Weitz · 发表于：Journal of the American Chemical Society · 年份：2025 · DOI：10.1021/jacs.5c04962 · 被引用次数：13 · 研究领域：Innovative Microfluidic and Catalytic Techniques Innovation、3D Printing in Biomedical Research、Electrowetting and Microfluidic Technologies

Lipase A (BsLipA) for improved thermotolerance. We screen a combinatorial library where 15 residues are saturated and identify multiple variants with 9-12 substitutions and >40 °C improvement in thermotolerance after a single round of mutagenesis. This platform offers a robust, scalable solution for protein engineering.