Effects of Lactobacillus plantarum-Fermented Feed on Growth and Intestinal Health in Haliotis discus hannai

作者：Ling Ke, Chenyu Huang, Peng Song, Mengshi Zhao, Fengqiang Lin, Zhaolong Li · 发表于：Microorganisms · 年份：2025 · DOI：10.3390/microorganisms13071603 · 被引用次数：8 · 研究领域：Aquaculture disease management and microbiota、Seaweed-derived Bioactive Compounds、Marine Bivalve and Aquaculture Studies

This study multidimensionally investigates the comprehensive effects of Lactobacillus plantarum (LP)-fermented feed on growth performance, intestinal health, and metabolic regulation in Pacific abalone (Haliotis discus hannai). The results demonstrate that LP fermentation significantly alters feed’s physical properties and nutritional profile, softening texture, increasing viscosity, and emitting an acidic aroma. Notably, it enhanced contents of cis-9-palmitoleic acid, α-linolenic acid (ALA), and functional amino acids (GABA, L-histidine, and L-asparagine), indicating that fermentation optimized ω-3 fatty acid accumulation and amino acid profiles through the modulation of fatty acid metabolic pathways, thereby improving feed biofunctionality and stress-resistant potential. Further analyses revealed that fermented feed markedly improved intestinal morphology in abalone, promoting villus integrity and upregulating tight junction proteins (ZO-1, Claudin) to reinforce intestinal barrier function. Concurrently, it downregulated inflammatory cytokines (TNF-α, NF-κB, IL-16) while upregulating anti-inflammatory factors (TLR4) and antioxidant-related genes (NRF2/KEAP1 pathway), synergistically mitigating intestinal inflammation and enhancing antioxidant capacity. Sequencing and untargeted metabolomics unveiled that fermented feed substantially remodeled gut microbiota structure, increasing Firmicutes abundance while reducing Bacteroidetes, with the notable enrichment of beneficial gen...