Omija (Schisandra chinensis) extract and soybean mixture to enhance memory function through the gut-brain axis: network pharmacology and in vivo validation of multi-target mechanisms

作者：Yue Yu, Hye Jeong Yang, Myung‐Sunny Kim, Ting Zhang, Chen Li, Min Jung Kim, Dai-Ja Jang, Sunmin Park · 发表于：Applied Biological Chemistry · 年份：2025 · DOI：10.1186/s13765-025-01019-4 · 被引用次数：3 · 研究领域：Tryptophan and brain disorders、Medicinal Plant Extracts Effects、Alzheimer's disease research and treatments

Abstract With the growing prevalence of memory impairment and limited treatment options, this study evaluated the cognitive-enhancing potential of an Omija ( Schisandra chinensis (Turcz). Baill) 50% ethanol extract and soybean mixture (OSM). By integrating network pharmacology with in vivo validation, we aimed to elucidate the molecular mechanisms and therapeutic efficacy of OSM. Network pharmacology was used to identify core pathways and targets associated with the memory-enhancing effects of Omija (gomisin, schizandrin) and soybean. To validate these findings, scopolamine-induced memory-impaired rats (2 mg/kg/day for 8 weeks) through the parasympathetic nervous system (PNS) suppression were administered OSM (100, 250, or 750 mg/kg/day) or dextrin (positive control) alongside a high-fat diet. Behavioral, metabolic, and molecular evaluations were conducted. Network analysis identified key targets involved in neuroinflammation including interleukin (IL)-17 and nuclear factor Kappa B (NF-κB) signaling and neurotransmitter regulation (dopamine synapse), including acetylcholinesterase (AChE), prostaglandin-endoperoxide synthase-2 (PTGS2), glycogen-synthase kinase-3beta (GSK-3β), solute carrier family 6 member 3 (SLC6A3; dopamine transporter), and plasminogen activator (PLAU). In vivo, OSM at 250 and 750 mg/kg significantly improved memory performance across passive avoidance, Y-maze, Morris water maze, and novel object recognition tests. These improvements were associated with su...