Moist‐Exposed Burn Ointment Promotes Angiogenesis During Full‐Thickness Wound Healing by Activating the SDF ‐1/ CXCR4 Axis in a Hyperglycaemic Rat Model

作者：Naili Zhang, Fu Li, Jiayuan Xu, Chengxu Gu, Hongxing Li, Dong Wang, Tongshen Liu, Xueying Shi, Ning Xu, Jun Qiu, Lina Ma · 发表于：Wound Repair and Regeneration · 年份：2025 · DOI：10.1111/wrr.70070 · 被引用次数：5 · 研究领域：Wound Healing and Treatments、Diabetic Foot Ulcer Assessment and Management、Pressure Ulcer Prevention and Management

The mechanism by which moist-exposed burn ointment (MEBO) promotes wound healing in diabetic foot ulcers (DFUs) remains unclear. To elucidate this mechanism, we investigated the role of stromal cell-derived factor-1 (SDF-1) in MEBO's ability to enhance ulcer healing and neovascularisation using a full-thickness wound model in hyperglycaemic rats. On post-wounding days 3, 7, and 14, the wound healing rates were significantly higher in the MEBO group compared to the model group and Vaseline group (p < 0.05). Additionally, the MEBO group exhibited increased epidermal layer thickness and enhanced collagen fibre deposition relative to the model group and Vaseline group (p < 0.05). Furthermore, the number of CD31-positive cells and microvascular density (MVD) were significantly elevated in the MEBO group compared to the model group and Vaseline group (p < 0.05). Flow cytometric analysis also demonstrated that the proportion of CD45-, CD34+, and VEGFR2+ cells in the wound area of the MEBO-treated group was significantly higher compared to the model group and Vaseline group. Expression levels of SDF-1, HIF-1α were also markedly higher in the MEBO group compared to the model group and Vaseline group (p < 0.05). Finally, a significant increase in double-positive CXCR4 and CD31 cells was observed exclusively in the MEBO group of hyperglycaemic rats (p < 0.05). These findings suggest that MEBO therapy promotes angiogenesis and accelerates wound healing through activation of the SDF-1/CXC...