Regulation of prognosis and tumor progression in cervical cancer by circ_0005728

作者：Shanshan Zhang, Xiyu Shen, Hongyin Cui · 发表于：Nucleosides Nucleotides & Nucleic Acids · 年份：2025 · DOI：10.1080/15257770.2025.2537168 · 研究领域：Circular RNAs in diseases、MicroRNA in disease regulation

To elucidate the prognostic significance and the underlying mechanism of action of circ_0005728 in patients diagnosed with cervical cancer (CCA). Intraoperative CCA and adjacent precancerous tissues from 208 patients were meticulously preserved at -80 °C. Kaplan-Meier survival curves were generated during a comprehensive 5-year postoperative follow-up. Cox analyses predicted the factors influencing prognostic progression in CCA patients. RT-qPCR detected the expression of circ_0005728. CCK8 and transwell observed cell proliferation, migration, and invasion. Flow cytometry recorded apoptotic changes. Dual luciferase reporter assay and RNA precipitation verified the interactions between circ_0005728 and miR-370-3p. High levels of circ_0005728 were observed in CCA tissues and cells. Elevated circ_0005728 expression and lymph node metastasis emerged as independent prognostic factors associated with poor outcomes in CCA patients. After silencing circ_0005728, cell functions were diminished and apoptosis increased. In addition, miR-370-3p is a downstream target gene of circ_0005728. Low expression of miR-370-3p was present in CCA tissues and was negatively correlated with circ_0005728 expression. The use of miR-370-3p inhibitor was able to induce cell proliferation, reduce apoptosis, and resist the reduction of cell migration and invasion caused by transfection of si-circ_0005728. The elevated expression of circ_0005728 in CCA patients was associated with prognostic mortality outco...