Optimizing the Electronic Structure of Iron Oxides via Nonmetallic Dopants for Enhanced Peroxidase Mimetic Catalysis

作者：Limin Ma, Ying Wang, Yaoyao Chen, Dongming Xu, Rui Han, Dongxu Jiao, Huanhuan Xing, Dewen Wang, Xiurong Yang · 发表于：ACS Nano · 年份：2025 · DOI：10.1021/acsnano.5c06779 · 被引用次数：22 · 研究领域：Advanced Nanomaterials in Catalysis、Electrochemical sensors and biosensors、Advanced biosensing and bioanalysis techniques

, thereby endowing the nanozymes with superior POD-like performance. Leveraging this insight, Bdoped Fe-based oxides (FOB) is successfully synthesized, demonstrating remarkable POD-like activity and ultrafast reaction kinetics. Mechanistic investigations revealed that B doping enhances electron transfer and intermediate adsorption by increasing the electron density and reducing the coordination number of the Fe center, concomitantly lowering the energy barrier for hydroxyl radical (·OH) formation and the rate-determining step. As a proof of concept, a three-enzyme cascade colorimetric biosensor integrating acetylcholinesterase (AChE)-choline oxidase (ChOx)-POD is constructed to perform ultrasensitive and selective detection of AChE activity and inhibitors. This study establishes a novel framework for designing high enzyme-mimicking performance transition-metal oxide nanozymes with doping nonmetallic atoms, provoking an inspiration for the rational design of nanozymes by regulating the electronic and coordination environment.