New functions of enzymatic cottonseed protein: Improvement of intestinal digestion and absorption, structure and microbial composition of juvenile yellow catfish (Pelteobagrus fulvidraco)

作者：Guangju Zhang, Wei‐Dan Jiang, Pei Wu, Yang Liu, Yao-Bin Ma, Hongmei Ren, Xiao‐Wan Jin, Weiwei Xiao, Ya Li, Xiao‐Qiu Zhou, Lin Feng · 发表于：Animal nutrition · 年份：2025 · DOI：10.1016/j.aninu.2025.03.022 · 被引用次数：2 · 研究领域：Aquaculture Nutrition and Growth、Protein Hydrolysis and Bioactive Peptides、Proteins in Food Systems

Enzymatic cottonseed protein (ECP) is a functional peptide product reported to have antioxidant and immunomodulatory properties. This study focused on how the intestinal apical junction complex (AJC) structure, microbial composition, growth performance, and digestive and absorptive capacity were affected by adding ECP to diets with a 3% protein reduction. A total of 1260 healthy juvenile yellow catfish weighing 5.90 ± 0.05 g were randomly assigned to seven experimental groups, each consisting of three replicates. a normal-protein (NP) diet (42%) or one of six low-protein (LP) diets (39%) with differing amounts of ECP (0%, 1%, 2%, 3%, 4%, and 5%, respectively) given for ten weeks. Dietary protein levels that were lowered by 3% (from 42% to 39%) disrupted the intestinal AJC structure and reduced growth performance, including specific growth rate (SGR, P = 0.042) and feed intake (FI, P = 0.001) and digestive and absorptive capacity, including the activities of alkaline phosphatase (AKP, P < 0.001), γ-glutamyl transpeptidase (γ-GT, P < 0.001), Na + /K + -ATPase ( P < 0.001), pepsin ( P < 0.001), lipase ( P = 0.009), amylase ( P < 0.001), chymotrypsin ( P < 0.001), and carboxypeptidase (CPs, P = 0.005). Adding 2% ECP to the LP diets (39%) improved SGR ( P = 0.001), FI ( P < 0.001), pepsin ( P < 0.001), the activities of γ-GT ( P < 0.001), AKP ( P < 0.001), lipase ( P < 0.001), amylase ( P < 0.001), Na + /K + -ATPase ( P < 0.001), chymotrypsin ( P < 0.001), Aps ( P < 0.001), and CP...