Biogeochemical Pathways of Phytate-P Utilization in Soil: Plant and Microbial Strategies

作者：Chenjing Liu, Ran Han, Chunyan Hu, Songge Deng, Xue Liu, Yanshan Chen, Benjamin L. Turner, Q. Lena · 发表于：Environmental Science & Technology · 年份：2025 · DOI：10.1021/acs.est.5c00724 · 被引用次数：14 · 研究领域：Phytase and its Applications、Plant Micronutrient Interactions and Effects、Iron Metabolism and Disorders

-inositol-1,2,3,4,5,6-hexakisphosphate) constitutes a large portion of the organic phosphorus in most soils, but its strong interactions with soil minerals and organic matter limit its availability to plants. Phytate can be used by plants only after it is desorbed from the soil matrix, with the inorganic P being released by phytases via cleavage of its phosphomonoester bonds. While plant phytases function primarily in its internal phytate remobilization, the role of microbial phytases in facilitating phytate-P utilization by plants remains poorly understood. This review focuses on phytase-producing plants and microbes and their uses in improving crop P acquisition from soil phytate. We discuss the behaviors of phytate and phytase in soils, especially their complex interactions with metal oxides, silicate minerals, and organic matter. Strategies to optimize soil phytase activity, including enzyme immobilization, site-directed mutagenesis, and rational protein design are also explored. Besides, we examine the mechanisms and hydrolysis pathways involved in phytase-mediated phytate hydrolysis, identifying situations where phytate utilization is limited by phytate solubility or phytase activity from an evolutionary perspective based on cultivation conditions and plant characteristics. Finally, we summarize strategies to increase plant utilization of soil phytate, including (1) amending soil with phytase-producing microbes, (2) expressing phytase gene in plant roots, (3) coupling p...