Total Glucosides of Paeony (TGP) Inhibits Macrophages Infiltration and NLRP3-Mediated Inflammatory Response in Acute Gouty Arthritis Mice

作者：Jing Liu, Ge Liu, Teng Chu, Yue Wu, Xingyu Yan, Liping Pang, Weirong Fang · 发表于：Journal of Inflammation Research · 年份：2025 · DOI：10.2147/jir.s527354 · 被引用次数：2 · 研究领域：Gout, Hyperuricemia, Uric Acid、Inflammasome and immune disorders、Rheumatoid Arthritis Research and Therapies

Purpose: The cascade of inflammation caused by the activation of NLRP3 inflammasome plays an important role in monosodium urate (MSU)-induced acute gouty arthritis (AGA) in mice. This study aimed to evaluate the therapeutic effect of total glucosides of paeony (TGP) in AGA mice and elucidate the underlying anti-inflammatory mechanism. Materials and Methods: MSU crystals were injected into the ankle joint to establish the AGA model in mice, and bone marrow-derived macrophages (BMDMs) were primary cultured and stimulated with MSU and LPS to model inflammatory conditions in vitro. Ankle diameter was measured to quantify joint swelling. Pain was assessed by the fifty percent paw withdrawal threshold (PWT) test and the bipedal support test. Pro-inflammatory cytokine levels and arthropathological damage were analyzed by ELISA, H&E staining, and immunohistochemistry staining. The mRNA and protein expression of NLRP3 inflammasome-associated signaling pathways were determined by RT-qPCR and Western blotting, respectively. Mitochondrial oxidative damage was evaluated using flow cytometry and immunofluorescence staining. Results: Four-week TGP administration with 360, 540, 720 mg/kg respectively significantly attenuated joint swelling and pain (P < 0.05, P< 0.01), reduced pro-inflammatory cytokine secretion (P < 0.01), and ameliorated joint pathology and macrophage infiltration (P < 0.01) in AGA mice. In BMDMs, TGP (30, 60 μg/mL) treatment notably suppressed mRNA and protein expression ...