Rapid detection of Hymenoscyphus fraxineus using genome‐specific primers based on loop‐mediated isothermal amplification assay

作者：Yu Zhou, Zan-Hua Zhu, Nicolas Feau, Yue Sun, Xu Liu, Wen Gao, Chongjuan You · 发表于：Pest Management Science · 年份：2025 · DOI：10.1002/ps.70012 · 被引用次数：1 · 研究领域：Forest Insect Ecology and Management、Insect Pheromone Research and Control、Fungal Plant Pathogen Control

BACKGROUND: Ash dieback, caused by Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz & Hosoya, is a serious fungal disease affecting Common ash (Fraxinus excelsior) populations across Europe and posing a global risk to ash trees worldwide. Traditional polymerase chain reaction (PCR)-based detection methods often target conserved gene regions, limiting the development of species-specific probes. However, the increasing availability of whole-genome sequences for H. fraxineus and its close relatives enables the identification of unique genes or genomic regions. RESULTS: This study utilized such genomic data to develop a sensitive, species-specific loop-mediated isothermal amplification (LAMP) assay for detecting H. fraxineus in Fraxinus mandshurica samples. The LAMP assay demonstrated a sensitivity of 2.53 fg/μL and proved effective in early detection of the pathogen. CONCLUSION: Altogether the developed LAMP detection method could be quite useful for further studies on the characteristics of the life cycle and colonization process of H. fraxineus in Asian ash host species. © 2025 Society of Chemical Industry.