Succession characteristics and assembly process of soil microbiome at reclaimed farmlands in coal mining area

作者：Jianhua Li, Zixu Li, Yanwen Sun, Jinjing Lu, Qiang Zhang, Xinhua He, Minggang Xu · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1633687 · 被引用次数：5 · 研究领域：Microbial Community Ecology and Physiology、Soil Carbon and Nitrogen Dynamics

Clarifying the succession patterns and assembly mechanisms of soil bacterial and fungal communities across reclamation chronosequences is essential for restoring soil health and ensuring ecological stability in mining areas. We analyzed soil microbial diversity, composition, co-occurrence network structure, and assembly processes using 16S rDNA/ITS sequencing and null models at 0 (R0), 1 (R1), 6 (R6), and 10 (R10) years of post-reclamation. Results showed that (1) Compared to R0, the R10 treatment resulted in significant increases in soil organic matter (SOM), total nitrogen (TN), available phosphorus (AP), and available potassium (AK) by 2.1-fold, 1.3-fold, 1.5-fold, and 0.4-fold, and also in activities of β-glucosidase (BG), N-acetyl-β-glucosaminidase (NAG), and leucine aminopeptidase (LAP), by 17-fold, 8.7-fold, and 1.8-fold, respectively ( p < 0.05). (2) Rising bacterial diversity (Shannon, Chao1) over time, contrasting with fungal diversity that declined initially before recovering. (3) As the reclamation progressed, the network complexity was increased for both bacteria and fungi, improving stability. The number of bacterial keystone taxa was first increased and then decreased, with Bacillota (formerly Firmicutes) being the dominant keystone phylum. Bacteroidetes, Proteobacteria, and Acidobacteria exhibited rapid temporal responses. The fungal keystone taxa increased progressively, with Ascomycota as the dominant keystone phylum, while Basidiomycota and Mortierel...