A short-chain dehydrogenase/reductase gene confers resistance to Verticillium wilt in cotton and reveals adaptive selection during domestication

作者：Mubashir Abbas, Muhammad Jawad Umer, Aamir Ali Abro, Menghan Zhang, Chao Lu, Qiankun Liu, Heng Wang, Mengying Yang, Yiman Liu, Huang Wenjuan, Muhammad Aamir Khan, Muhammad Abid, Muhamad Askari, Muhammad Aneeq Ur Rahman, Yuqing Hou, Jie Zhang, Yanchao Xu, Xiaoyan Cai, Zhongli Zhou, Rui Zhang, Fang Liu · 发表于：Plant Stress · 年份：2025 · DOI：10.1016/j.stress.2025.100971 · 被引用次数：1 · 研究领域：Research in Cotton Cultivation、Cocoa and Sweet Potato Agronomy、Plant Virus Research Studies

Cotton is a globally important fiber crop that faces significant yield losses due to Verticillium wilt caused by Verticillium dahliae . Short-chain dehydrogenase/reductases (SDRs) are NAD(P)(H)-dependent enzymes involved in metabolic pathways and stress responses, but their role in disease resistance is not well understood in cotton. The objective of this study was to investigate the function of GhSDR500 in cotton defense against V. dahliae . A combination of population genetics, pangenome analysis, gene expression profiling, transcription factor binding analysis, and functional validation through Virus-Induced Gene Silencing (VIGS) was employed. Population genetic analysis revealed a favorable GhSDR500 allele selected during cotton domestication. A G>C mutation causing a glycine-to-alanine substitution in the NAD domain was identified, with the G allele strongly favored in modern cultivars due to its association with enhanced resistance. Pangenome analysis further highlighted presence–absence variations and transposable elements around GhSDR500 . Expression studies showed strong upregulation of GhSDR500 in resistant cotton varieties during early infection stages. VIGS confirmed its role in disease resistance, as silencing GhSDR500 increased susceptibility to V. dahliae and reduced expression of pathogenesis-related genes. Transcription factor binding analysis identified WRKY genes as key regulators. These results demonstrate that GhSDR500 plays a key role in resistance to V....