Ultrasound assisted homing of human umbilical cord mesenchymal stem cells promotes recovery from acute respiratory distress syndrome

作者：Bei-Ying Wang, Xiao Zhang, Tao Li, Wei-Wei Qin, Xiang Liu, Kaijun Lu, Lixin Sun, Wei Han · 发表于：Stem Cell Research & Therapy · 年份：2025 · DOI：10.1186/s13287-025-04545-6 · 被引用次数：2 · 研究领域：Ultrasound and Hyperthermia Applications、Mesenchymal stem cell research、Ultrasound in Clinical Applications

BACKGROUND: Human umbilical cord mesenchymal stem cells (hUC-MSCs) show potential for treating acute respiratory distress syndrome (ARDS), however, their homing to the lungs and survival time are insufficient. In this study, we evaluated whether pulsed focus ultrasound (pFUS) could promote the homing and prolonged retention of hUC-MSCs in the lungs of ARDS mice and explored the mechanisms involved. METHODS: Mice were divided into four groups: the NC group, the LPS group, the MSCs group, and the pFUS + MSCs group. Except for the NC group, the other three groups were constructed as ARDS models and given PBS, MSCs and pFUS + MSCs interventions. hUC-MSCs were used to assess lung tissue injury by HE staining, inflammatory cell count in alveolar lavage fluid (BALF), and expression of Tnf, Il1b and Il6 in the lung tissues; and apoptosis and proliferation in the lung tissues were assessed by TUNEL and immunofluorescence. Bioluminescence imaging was used to detect the homing rate and survival of hUC-MSCs in mouse lungs from 1 to 7 days. Cxcl5 and Igf1 was found to be differentially expressed and highly enriched by mRNA sequencing in MSC and sonicated groups and verified by PCR combined with ELISA. RESULTS: Compared with the LPS group, the lung inflammatory infiltrate and lung tissue damage in the MSCs group and pFUS + MSC group were alleviated, the number of inflammatory cells in the BALF and the expression of Tnf, Il1b and Il6 in the lung tissues were reduced, the expression of TUNEL...