A synbiotic combination of mixed probiotics and oligofructose restores intestinal microbiota disturbance in DSS-induced colitis in mice

作者：Weiwei Ma, Lian Lian, Lidong Guo, Yanan Wu, Lili Huang · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1582155 · 被引用次数：10 · 研究领域：Gut microbiota and health、Inflammatory Bowel Disease、Probiotics and Fermented Foods

Both probiotics and prebiotics can regulate gut microbiota and produce metabolites that are beneficial to the host, showing potential therapeutic prospects for ulcerative colitis (UC). This study aimed to investigate the therapeutic effects and mechanisms of low-, medium-, and high-dose probiotic mixtures ( Bifidobacterium animalis subsp. lactis XLTG11: Lactobacillus paracasei Glory LP16: Lactiplantibacillus plantarum CCFM8661) combined with oligofructose (FOS) on DSS-induced colitis mice. Male BALB/c mice were induced with 2.5% DSS for colitis and then treated with low-, medium-, and high-dose mixed probiotics and oligofructose synbiotics (6 × 10 6 CFU probiotics + 1.5 mg FOS synbiotics/day, 6 × 10 7 CFU probiotics + 1.5 mg FOS synbiotics/day, and 6 × 10 8 CFU probiotics + 1.5 mg FOS synbiotics/day) for 1 week. Colonic inflammation was evaluated by body weight, diarrhea index, immune organ index and colon length; the protective effect of synbiotics on the intestinal barrier was evaluated by HE staining, ELISA and RT-qPCR to detect the degree of intestinal tissue damage, inflammatory cytokines, intestinal permeability and tight junction proteins. In addition, the intestinal microbiota and short-chain fatty acids (SCFAs) were estimated by metagenomics and LC-MS, respectively, to analyze the mechanism of action of synbiotics in alleviating colitis. The results showed that the combination of mixed probiotics and oligofructose could significantly alleviate the symptoms of colitis...