Epidemiological and molecular investigation of a Salmonella Agona-associated foodborne outbreak in Taiyuan, Shanxi Province, China, 2022

作者：Ji‐Tao Wang, Weidong Zheng, Yuezhen Li, Jihong Xu, Xiaomei Jia, Lin Miao, Junhong Wu, Ping Zhang, Tao An, Xu Yang, Lirong Liang, Xiaohua Zhang · 发表于：BMC Infectious Diseases · 年份：2025 · DOI：10.1186/s12879-025-11305-7 · 被引用次数：2 · 研究领域：Salmonella and Campylobacter epidemiology、Listeria monocytogenes in Food Safety、Bacillus and Francisella bacterial research

BACKGROUND: From July 23 to 24, 2022, Qingxu People's Hospital in Taiyuan reported an increase in acute gastroenteritis cases among individuals who had dined together.An investigation was initiated to identify factors associated with acute intestinal infection and to prevent further illness. METHODS: To isolate, cultivate, and identify pathogens, we collected samples from patients, food, and the environment. The isolated strains underwent multiple tests, including antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE), and whole-genome sequencing (WGS). Based on the WGS data, analyses were conducted, including serotype prediction, resistance gene prediction, multilocus sequence typing (MLST), core genome multilocus sequence typing (cgMLST), single nucleotide polymorphism (SNP) analysis, and phylogenetic analysis. RESULTS: A total of 11 samples were collected from Patient 1's residence and the supermarket. These included 2 samples of leftover food from a family gathering, 4 samples of the supermarket environment, and 5 stool samples from 5 patients. Among these, two strains of Salmonella were isolated from the food, and three strains were isolated from the patients' stools. All five strains of Salmonella were identified as Salmonella Agona (S. Agona) and were susceptible to the 15 antibiotics tested. The PFGE banding patterns were identical among the strains. Bioinformatics analysis revealed that the isolated strains were all S. Agona of sequence type ST...