Gastric cancer adapts high lipid microenvironment via suppressing PPARG-FABP1 axis after arriving in the lymph node

作者：Yinan Liu, Liang Tang, Baifu Peng, Shaoji Zhao, Ziling Shao, Kaiyu Sun, Jinning Ye, Wei Chen, Jianbo Xu · 发表于：Redox Biology · 年份：2025 · DOI：10.1016/j.redox.2025.103759 · 被引用次数：4 · 研究领域：Ferroptosis and cancer prognosis、Cancer, Lipids, and Metabolism、Immune cells in cancer

AIM: Gastric cancer (GC) primarily metastasizes through lymphatic channels, although lymphatic metastasis remains relatively inefficient. Changes in cellular metabolism, known as metabolic reprogramming, plays a significant role in the adaptive survival of cells during the process. Therefore, understanding the mechanism underlying metabolic reprogramming in lymph node (LN) metastasis is crucial for the development of targeted therapies for advanced gastric cancer. This study aimed to investigate the metabolic adaptations of GC cells during LN metastasis, with a particular focus on lipid metabolism reprogramming. METHODS: Non-targeted lipidomic sequencing, combined with tumor cell flow sorting and RNA sequencing, was used to explore differences in lipid microenvironments and changes in lipid metabolism pathways between lymph nodes and primary tumors. Single-cell sequencing data were analyzed to confirm these results. Transmission electron microscopy, BODIPY 581/591 staining, and ferroptosis inhibitors were used to confirm the effects of arachidonic acid (AA) on ferroptosis sensitivity in gastric cancer. Public databases and ChIP-qPCR tests were used to investigate the role of PPARγ pathway in regulating FABP1 transcription. RESULTS: Lipid metabolism pathway was inhibited following lymph node metastasis, with reduced lipid catabolites observed in the lymph nodes. Single-cell data also supported these findings. Physiological concentrations of AA were shown to increase ferroptosi...