Effect of Marine‐Derived Scallop Peptide Hydrolysate on Immune Modulation and Gut Microbiota Restoration in Cyclophosphamide‐Induced Immunosuppressed Mice

作者：Muhammad Ilyas, Mujeeb Ur Rahman, Muhsin Ali, Ting Deng, Nabeel Ahmed Farooqui, Sharafat Ali, Hidayat Ullah, Yamina Alioui, Renzhen Ma, Shuming Lu, Liang Wang, Yi Xin · 发表于：Food Science & Nutrition · 年份：2025 · DOI：10.1002/fsn3.70421 · 被引用次数：7 · 研究领域：Protein Hydrolysis and Bioactive Peptides、Infant Nutrition and Health、GDF15 and Related Biomarkers

ABSTRACT Cyclophosphamide (CTX), a chemotherapy agent, can weaken immune responses and disrupt gut microbiota, resulting in dysbiosis and inflammation. The gut microbiota, a composite community of microorganisms, plays a central role in regulating metabolism, supporting biological functions, and maintaining immune balance. Dysbiosis may lead to various systemic diseases and metabolic issues. This underscores the importance of immunomodulators to boost immune health and restore balance during CTX treatment. This study assessed the impact of scallop peptide hydrolysate (SCH), derived from scallops collected from a local seafood market in Dalian, China, on immune suppression, intestinal integrity, and gut microbiota composition in BALB/c mice with CTX‐induced immunosuppression. The findings demonstrated that SCH significantly restored intestinal mucosal integrity of goblet cells, enhanced the levels of tight‐junction proteins (Occludens, Zonula‐1, Mucin‐2, Claudin‐1), and improved the histological state of both the colon and spleen. SCH treatment resulted in increased body weight, improved immune organ indices, and enhanced levels of pro‐inflammatory cytokines and immunoglobulins, while reducing anti‐inflammatory cytokines. Furthermore, SCH exhibited no cytotoxic effects on RAW264.7 macrophages, promoting the formation of pseudopodia and enhancing phagocytic activity, thereby improving cell‐mediated immunity and splenic lymphocyte proliferation. Moreover, SCH therapy markedly in...